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The hematopoietic microenvironment provides a complex molecular milieu that regulates the self-renewal and differentiation activities of stem cells. We have characterized a stem cell supportive cell line, AFT024, derived from the murine fetal liver microenvironment. Highly purified in vivo transplantable mouse stem cells are maintained in AFT024 cultures at input levels, while other primitive progenitors are expanded. In addition, human stem cells are very effectively supported by AFT024. We suggest that AFT024 represents a component of an in vivo stem cell niche. To determine the molecular signals elaborated in this niche, we undertook a functional genomics approach that coalesces extensive sequence mining of a subtracted cDNA library, high-density array hybridization and in-depth bioinformatic analyses. The data have been assembled into a biological process oriented database, and represent the first molecular profile of a candidate stem cell niche.
This web site was released with the publication of: A Molecular Profile of a Hematopoietic Stem Cell Niche (Jason A. Hackney, Pierre Charbord, Brian P. Brunk, Christian J. Stoeckert, Jr., Ihor R. Lemischka, and Kateri A. Moore., Proc. Natl. Acad. Sci. USA, Vol. 99, Issue 20, 13061-13066, October 1, 2002). Published online before print September 11, 2002, 10.1073/pnas.192124499; http://www.pnas.org. PMID: 12226475. Download the complete sequence set in zipped FASTA format.