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Cytokine RNA Expression in Stromal Cell Lines

 

2018

AFT024

2012

CFC034

BFC012

MC3T3PA6

NIH3T3

Flk2/Flt3-L

++

++

++

++

++

+

+

cKit-L

++++

++++

++++

++++

++++

++

++++

LIF

++

++

++

++

++++

+

-

Il-6

+++

+

+++

+++

++++

+

-

Il-7

-

-

-

-

-

-

-

Il-11

+++

++++

+++

+

+++

+

++

G-CSF

+

+

+

+

+

-

-

GM-CSF

++

+

+

+

+

-

-

IGF-1

+++

++++

+++

++++

++++

+

+

TGF-b

++++

++++

++++

++++

++++

+++

+++

MIP-1a

-

-

-

-

-

-

-

MPL-L

+

++

+++

+

+++

+

+++

Random hexamer primed cDNAs were synthesized using reverse transcriptase SuperScript II (Life Technologies) from cell line RNA. PCR amplification with appropriate primers was done for 30R. Results were interpreted from intensity of ethidium bromide staining of amplified products.