Table A

Clone ID	Size (bp)	AFT024	2018	2012	BFC012	2058
1	1900	+	+	ND	ND	ND
5	1600	++	+	ND	ND	ND
9	400	++	+	ND	ND	ND
13	1000	++	+	ND	ND	ND
14	1800	++	+	ND	ND	ND
32	300	++	+	ND	ND	ND
54	200	+	+	ND	ND	ND
67	1600	+	+	ND	ND	ND
68	250	+	+	ND	ND	ND
69	1600	+	+	ND	ND	ND
83	560	+	+	ND	ND	ND
A-15	1350	++	+	ND	ND	ND
A-19	610	++	+	ND	ND	ND
A-24	590	++	+	ND	ND	ND
A-25	200	+	+	ND	ND	ND
A-27	500	+	+	ND	ND	ND
A-33	1000	+	+	ND	ND	ND
B-7	1100	+	+	ND	ND	ND
B-11	1600	++	+	ND	ND	ND
B-12	570	++	+	ND	ND	ND
B-14	580	++	+	ND	ND	ND
B-20	300	+	+	ND	ND	ND
B-36	580	+	+	ND	ND	ND
B-17	1200	++++	-	ND	ND	ND
B-1 (AFTP1)	1700	++++	-	++	-	+
A-21 (dlk)	580	++++	-	+++	-	-
82 (Fibulin-3)	1900	++++	+++	+++	+	+++
59 (Osteoglycin)	1270	++	-	+++	-	ND
23 (Lysyl oxidase)	2000	++	-	++++	+	+++

Original strip Northerns done with AFT024 and 2018 to validate the subtracted library. The last 5 AFT024 overexpressed clones were studied in all stromal cell lines and are in StroCDB. 17/29 (59%) show a higher level of expression in AFT024 compared to 2018. These clones were sequenced by hand prior to size selection of the subtracted library.
 
 

Table B

Clone ID	Size (bp)	AFT024	2018	2012	BFC012	2058
F77 (AFTP2)	1070	++++	-	+	-	-
C82	2800	+	-	++	-	+++
B77	1350	++	+	++	+	+
B91	1600	++	+	+++	+++	++++
A14	1700	+++	-	+++	+	+++
F26	1540	++++	-	++	+	++
E25	1545	+++	-	++	+	-
F71	1600	++	+	+	+	+++
D77	1760	+	++	+	+	+++
A79	1250	+++	++	++	+	+++
LL6in10039	2200	+++	-	+++	+	++++
LL6in10848	1800	+++	+	+	+	+
LL6in10953	1500	+++	-	++++	+	-
LL6in10110	1600	+++	++	++++	++++	+
LL6in11084	2800	+	-	++	-	-
LL6in12362	2000	+++	-	+++	-	-
LL6in11125	1500	++++	-	+++	++	+++
LL6in12229	2000	+++	++	+++	+	++++
LL6in12291	1500	+++	+	++	++	ND
LL6in12232	1400	+++	++	++++	-	+
LL6in12249	2900	++++	+	+++	-	+++
LL6in11228	1900	++	-	++++	-	+++
LL6in10198	1580	+++	+	+++	+	+
LL6in10040	1900	++	-	++++	-	+++
LL6in11199	3000	++	-	+++	+	++
LL6in11564	2900	++++	++	++++	+	++
LL6in11648	2000	+++	++	++++	+	++
LL6in10139	1500	+	-	+	-	-
LL6in11935	2900	+++	-	++++	-	-
LL6in10314	2800	++++	+	-	-	-
LL6in11214	2800	+	-	++++	-	-

All cDNAs were chosen for expression analysis based on the bioinformatic analysis and annotation that revealed their protein name, category, and/or interesting motifs, transmembrane domains or signal peptides in novel proteins. A-E clones were sequenced by CBI. All LL6in1xxxx clones were sequenced by Incyte after size selection of the subtracted library. 29/31 cDNAs are more highly expressed in AFT024 compared to 2018. Combining both tables the 46/60 cDNAs are more highly expressed in AFT024 compared to 2018 for 76.7%. There is a striking correlation in expression of these cDNAs by AFT024 and 2012, both stem cell supporting stromal cell lines compared to the two non-supporting lines 2018 and BFC012. The average insert size of the cDNAs from the sized selected library is 2118+627 compared to 1155+657 from the non-sized selected library.