Class	Function	Protein	Gene Name	Detected in^**	Sequence^§	PW^***	StroCDB ID
protein	collagen synthesis	lysyl oxidase	LOX2/LOXL	DB, A, GM	em	2, 9	A03
protein	collagen synthesis	lysyl hydroxylase	PLOD	DB	homol	4, 9	LL6in10596T7
protein	collagen synthesis	dermatopontin/tyrosine rich acidic matrix protein/early quiescence protein	DPT	DB, A, GM	em	7
protein	major interstitial collagen	collagen I [pro alpha2(I) chain precursor]	COL1A2	DB, A, GM	em	3, 4, 8	LL6in11742T7
protein	fibrillar collagens	collagen V [(pro alpha3(V)]	COL5A3	DB	em	3, 4	LL6in21457
protein	fibrillar collagens	collagen VI [(pro alpha3(VI)]	COL6A3	DB	em	2, 3, 5, 8	LL6in22399
protein	anchoring fibril collagen	collagen VII [(pro alpha1(VII)]	COL7A1	DB	em	6	LL6in21827
protein	short chain collagen	collagen VIII [pro alpha1(VIII)]	COL8A1	A
protein	multiplexin collagen	collagen XVIII [pro alpha1 (XVIII)]	COL13A1	DB	em		LL6in22029
protein	collagen-binding protein	TGF-beta induced gene product-beta ig-h3	TGFBI/BIGH	A		1
protein	collagen-binding protein	osteoblast specific factor 2/fasciclin I-like	OSF2	A		3, 6
protein	collagen-binding protein	matrilin-2	MATN2	A
protein	adhesive glycoprotein	fibronectin	FN1	DB, A	em	1, 3, 4	LL6in23280
protein	adhesive glycoprotein	fibulin 1	FBLN1	DB	em	3, 6, 9	LL6in20799
protein	adhesive glycoprotein	fibulin 3	EFEMP1/FBLN3	DB	em		82-10finseq
protein	matrix-attached adhesion protein	thrombospondin 2	THBS2/ TSP2	A
protein	basement membrane	F-spondin/mindin precursor	-	DB	homol		LL6in20225
protein	basement membrane	entactin-2/nidogen-2	NID2	DB, GM	em	9	LL6in12272T7
protein	secreted anti-adhesive protein	SPARC-like1	SPARCL1/SC1	DB	em		LL6in11260T7
protein		cochlin precursor (COCH-5B2)	COCH	DB	em	6	LL6in11111T7
protein		polydomain protein	POLYDOM	DB	em		C82
proteoglycan	hyaluronan synthesis	hyaluronan synthase	HAS2	DB	em		LL6in11422T7
proteoglycan	hyaluronan degradation	lysosomal hyaluronidase	HYAL2	DB	em		LL6in10295T7
proteoglycan	heparan sulfate synthesis	heparan sulfate 6-O-sulfotransferase 1	HS6ST	DB	em		LL6in20389
proteoglycan	Small Leucine Rich Proteoglycan	biglycan	BGN	DB, A, GM	em		LL6in10018T7
proteoglycan	Small Leucine Rich Proteoglycan	decorin	DCN	DB	em	3, 4, 8	LL6in20096
proteoglycan	Small Leucine Rich Proteoglycan	osteoglycin/mimecan/osteoinductive factor	OGN/OIF	DB, A	em		59
proteoglycan	hyalectan	chondroitin sulfate PG core protein 2/versican	CSPG2	DB, A	em	6	LL6in10771T7
proteoglycan	secreted proteoglycan	proteoglycan-4 (PRG-4)	BG174L6.2	DB, A	homol		LL6in10770T7
proteoglycan	membrane proteoglycan	syndecan-3	SDC3	A		8
proteoglycan	associated to perlecan	extracellular matrix protein 1	ECM1	GM	em	3, 4, 7
protease	matrix metalloprotease (MMP)	matrix metalloprotease-9/gelatinase B	MMP9	DB	em		LL6in11570T7
protease	serine protease	fibroblast-activation protein	FAP	DB	em		LL6in20256
protease	serine protease inhibitor	plasminogen activator inhibitor type 1	PAI1	A		4, 9
protease	serine protease inhibitor	plasminogen activator inhibitor type 2	PAI2	DB	em	9	LL6in10022T7
protease	serine protease inhibitor	late gestation lung protein	LGL1	DB	homol		LL6in12264T7
protease	cysteine protease	cathepsin B	CTSB	A		3, 8
protease	cysteine protease	cathepsin K	CTSK	DB, A	em	9	LL6in10211T7
protease	cysteine protease	cathepsin L	CTSL	DB	em		B08
protease	a disintegrin and MMP domain (ADAM)	a disintegrin and MMP domain-8	ADAM8/CD156	DB	em		LL6in20858
protease	a disintegrin and MMP domain (ADAM)	a disintegrin and MMP domain-9	ADAM9/MDC9	DB	em	9	LL6in10008T7
protease	a disintegrin and MMP domain (ADAM)	a disintegrin and MMP domain-23	ADAM23	DB	em		LL6in20985
protease	ADAM with thrombospondin motifs (ADAMTS)	aggrecanase-1	ADAMTS4	DB	em		LL6in22782

** DB : StroCDB (database AFT024-2018) at http://stromalcell.princeton.edu; A : Affimetrix chips; GM : GM chips

*** PW : already given in previous works on gene product screening using stromal cells (shown in italic):

§ for gene products in the database: em = exact match; homol = homolog

1. Dieudonne SC, Kerr JM Xu T et al. Differential display of human marrow stromal cells reveals unique mRNA expression patterns in response to dexamethasone.J Cell Biochem. 1999;76 : 231-43.

2. Locklin RM, Riggs BL, Hicok KC et al. Assessment of gene regulation by bone morphogenetic protein 2 in human marrow stromal cells using gene array technology. J Bone Miner Res. 2001; 16 : 2192-204.

3. Tremain N, Korkko J, Ibberson D et al. MicroSAGE analysis of 2,353 expressed genes in a single cell-derived colony of undifferentiated human mesenchymal stem cells reveals mRNAs of multiple cell lineages. Stem Cells. 2001; 19 :408-18.

4. Jia L, Young MF, Powell J et al. Gene expression profile of human bone marrow stromal cells: high-throughput expressed sequence tag sequencing analysis. Genomics. 2002; 79 : 7-17

5. Doi M, Nagano A, Nakamura Y. Genome-wide screening by cDNA microarray of genes associated with matrix mineralization by human mesenchymal stem cells in vitro. Biochem Biophys Res Commun. 2002; 290 : 381-90

6. Graf L, Iwata M, Torok-Storb B. Gene expression profiling of the functionally distinct human bone marrow stromal cell lines HS-5 and HS-27a. Blood. 2002; 100 : 1509-11.

7. Sekiya I, Vuoristo JT, Larson BL, Prockop DJ. In vitro cartilage formation by human adult stem cells from bone marrow stroma defines the sequence of cellular and molecular events during chondrogenesis. Proc Natl Acad Sci U S A. 2002; 99 : 4397-402.

8. Wieczorek G, Steinhoff C, Schulz R et al. Gene expression profile of mouse bone marrow stromal cells determined by cDNA microarray analysis. Cell Tissue Res. 2003; 311 : 227-37.

9. Qi H, Aguiar DJ, Williams SM et al. Identification of genes responsible for osteoblast differentiation from human mesodermal progenitor cells. Proc Natl Acad Sci U S A. 2003; 100 : 3305-10.

Table 4 : Extra-Cellular Matrix (ECM) Molecules

From Gene expression in stem cell-supporting stromal cell lines. (Ann N Y Acad Sci. 2005 Jun;1044:159-67. Charbord P, Moore K)