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Table 7: G Protein Coupled-Receptors (GPCR), Migration Cues (MC),
Eicosanoids, Wnt, Vaso-Active Peptides (VAP)

From Gene expression in stem cell-supporting stromal cell lines. (Ann N Y Acad Sci. 2005 Jun;1044:159-67. Charbord P, Moore K)

CategoryClassFunctionProteinGene nameDetected in**Sequence§PW***StroCDB ID
Eicosanoid Modulator PLA2 activation phospholipase A2-activating protein PLAA/PLAP DB em LL6in22674
Eicosanoid Modulator PGh1-->PGE2 prostaglandin E synthase PTGES DB em LL6in22218
Eicosanoid Modulator PGh1-->PGI2 prostacyclin synthase (PGIS) PTGIS DB, A em LL6in21778
Eicosanoid Modulator PAF-->lysoPAF platelet-activating factor (PAF) acetylhydrolase PLA2G7 DB em LL6in12266T7
Eicosanoid Transporter ATP-dependent PGE uptake prostaglandin transporter/PGT SLC21A2 DB, GM em LL6in10294T7
wnt wnt organogenesis wnt-5a WNT5A DB em LL6in11292T7
wnt wnt organogenesis wnt-10B WNT10B/WNT12 A
wnt wnt organogenesis Dickkopf -3 DKK3 DB em LL6in21996
wnt Receptor GPCR polarity gene (frizzled) FZD1 DB homol LL6in12103T7
wnt Receptor organogenesis LDL-receptor related protein 6 LRP6 DB em LL6in10590T7
wnt TF organogenesis transcription factor-3 TCF3 DB em LL6in20817
MC MC attraction/repulsion semaphorin Y SEMA6C/SEMAY DB em B77
MC MC attraction/repulsion netrin receptor homolog UNC5H2 DB homol B91
MC MC attraction/repulsion ephrin A2 EFNA2 DB em LL6in22969
MC Receptor attraction/repulsion plexin 3 PLXNA3 A em
MC Receptor attraction/repulsion rig-1 (roundabout family) RBIG1 DB em LL6in10848T7
GPCR Receptor hormone ligand parathyroid hormone(PTH)/PTH-related peptide receptor (PTHR1) PTHR DB em 7, 9 LL6in11114T7
GPCR Transducer GPCR signalling G protein beta-subunit GNB1 A
GPCR Transducer GAP for Ga subunit G protein signaling regulator RGS2 RGS2 A
GPCR Transducer GAP for Ga subunit G protein signaling regulator RGS3 RGS3 DB em C16
GPCR Transducer GAP for Ga subunit G protein signaling regulator RGS4 RGS4 DB em 6 LL6in11416T7
GPCR Transducer GAP for Ga subunit G protein signaling regulator RGS5 RGS5 DB em LL6in10925T7
GPCR Transducer downstream of Ga adenylyl cyclase type VI ADCY6 DB, A em LL6in21769
GPCR Transducer downstream of Ga adenylyl cyclase type VII ADCY7 A
GPCR Transducer A-kinase cAMP-dependent protein kinase type I-beta regulatory chain PRKAR1B A
GPCR Transducer target of A-kinase LIM and Sh4 domain-containing protein-1 LASP1 A
VAP VAP vasoconstriction (AT precursor) angiotensinogen precursor AGT DB em LL6in11850T7
VAP Receptor GPCR; relaxation via NO muscarinic acetylcholine receptor M5 CHRM5 DB homol A86
VAP Receptor GPCR; relaxation via NO/PGI2 bradykinin B2 receptor BDKRB2 DB em LL6in10279T7
VAP Modulator desensitization of B2 bradykinin R beta-adrenergic receptor kinase 1 ADRBK1 DB em LL6in10495T7
VAP Modulator generation of angiotensin (AT)-(1-7) prolyl endopeptidase PREP/ PEP DB em LL6in10491T7
VAP Modulator modulation of of AT signalling angiotensin II, type I receptor-associated protein AGTRAP DB em LL6in11392T7
VAP Modulator modulation of of AT signalling SM-20 (growth factor-responsive smooth muscle protein) - DB homol LL6in20916
VAP Modulator generation of endothelin endothelin-converting enzyme-2 ECE2 DB homol LL6in20554

** DB : StroCDB (database AFT024-2018) at http://stromalcell.princeton.edu; A : Affimetrix chips; GM : GM chips

*** PW : already given in previous works on gene product screening using stromal cells (shown in italic):

§ for gene products in the database: em = exact match; homol = homolog

1. Dieudonne SC, Kerr JM Xu T et al. Differential display of human marrow stromal cells reveals unique mRNA expression patterns in response to dexamethasone.J Cell Biochem. 1999;76 : 231-43.

2. Locklin RM, Riggs BL, Hicok KC et al. Assessment of gene regulation by bone morphogenetic protein 2 in human marrow stromal cells using gene array technology. J Bone Miner Res. 2001; 16 : 2192-204.

3. Tremain N, Korkko J, Ibberson D et al. MicroSAGE analysis of 2,353 expressed genes in a single cell-derived colony of undifferentiated human mesenchymal stem cells reveals mRNAs of multiple cell lineages. Stem Cells. 2001; 19 :408-18.

4. Jia L, Young MF, Powell J et al. Gene expression profile of human bone marrow stromal cells: high-throughput expressed sequence tag sequencing analysis. Genomics. 2002; 79 : 7-17

5. Doi M, Nagano A, Nakamura Y. Genome-wide screening by cDNA microarray of genes associated with matrix mineralization by human mesenchymal stem cells in vitro. Biochem Biophys Res Commun. 2002; 290 : 381-90

6. Graf L, Iwata M, Torok-Storb B. Gene expression profiling of the functionally distinct human bone marrow stromal cell lines HS-5 and HS-27a. Blood. 2002; 100 : 1509-11.

7. Sekiya I, Vuoristo JT, Larson BL, Prockop DJ. In vitro cartilage formation by human adult stem cells from bone marrow stroma defines the sequence of cellular and molecular events during chondrogenesis. Proc Natl Acad Sci U S A. 2002; 99 : 4397-402.

8. Wieczorek G, Steinhoff C, Schulz R et al. Gene expression profile of mouse bone marrow stromal cells determined by cDNA microarray analysis. Cell Tissue Res. 2003; 311 : 227-37.

9. Qi H, Aguiar DJ, Williams SM et al. Identification of genes responsible for osteoblast differentiation from human mesodermal progenitor cells. Proc Natl Acad Sci U S A. 2003; 100 : 3305-10.


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