Web-only Data

Supplementary Tables

From Gene expression in stem cell-supporting stromal cell lines.
(Ann N Y Acad Sci. 2005 Jun;1044:159-67. Charbord P, Moore K)

• Transcription Factors (table)
• Membrane Proteins (table)
• Cytoskeleton (table)
• Extracellular Matrix (table)
• Cell Adhesion Molecules (table)
• Cytokines and Chemokines (table)
• Regulators (table)

Supplementary Data

• Generation of stromal cell lines (Image, Ref)
• Results of screen for in vitro stem cell maintenance (Image)
• In vitro maintenance of stem cell repopulating ability by co-culture with stromal cell lines (Image A, Image B)
• Single-cell deposition experimental design (Image)
• Cellular content of a single-cell derived cobblestone area (Image)
• Developmental potential of single stem cells cultured on AFT024 (Image)
• RT-PCR expression screen of common regulatory cytokines (Image)
• Tabulated results from the cytokine expression screen (Image)
• Retroviral marking strategy (Image)
• PCR analysis of integrated proviral DNA (Image)
• Expression screen by northern blot analysis (Image)
• Library statistics and complexity analysis (Image)
• Clusters generated from the StroChip experiment (Image)
• Clusters generated from the membrane array experiment (Image)

Additional Biological Data

Quiescent Sca-1^pos, cKit^pos, Lineage^neg, Rhodamine lo bone marrow cells

• Rhodamine lo and hi sort profiles (Image)
• Rhodamine lo repopulating frequency by limiting dilution (Image)
• Lineage content of rhodamine lo repopulating stem cells (Image)
• CFU-C progenitors in Rhodamine lo and hi cells (Image)
• Expansion of CFU-C progenitors in AFT024 co-cultures (Images)
• Limiting dilution analysis of Cobblestone Area Forming Cells (CAFC) from Rhodamine lo and hi cells on AFT024 (Image)
• Percent frequency of CAFC from Rhodamine lo and hi cells on AFT024 (Image)
• Single cell frequency of Rhodamine hi cells (Image)
• Single cell frequency of Rhodamine lo cells (Image)
• Primary and Secondary CAFC frequency of Rhodamine lo cells on different stromal cell lines (Image)
• Maintenance of transplantable Rhodamine lo stem cell activity on AFT024 (Image)

Double Sorted AA4.1^pos, Sca-1^pos, cKit^pos, Lineage^neg/lo day 14.5 fetal liver cells

• Flow profile of double sorted cells (Image)
• Limiting-dilution analysis of competitive repopulating stem cell activity (CRSCA) of double sorted cells and their lineage content (Image)
• Limiting-dilution analysis of CAFC from double sorted cells on AFT024 (Image)
• Percent frequency of primary and secondary CAFC on AFT024 of double sorted fetal liver stem cells (Image)